Coding

Part:BBa_K4378004:Design

Designed by: Sigurður Smári Davíðsson   Group: iGEM22_Uppsala   (2022-10-06)

TphA1, a cognate reductase of TPADO


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Design Notes

During the design of this part, one internal EcoRI site was mutated out in silico prior to synthesis. Additionally this part has been codon optimized for E. coli.

Source

Sequence originates from Burkholderia xenovorans, the sequence published here was adapted from the following publication:

Bains J, Wulff JE, Boulanger MJ. 2012. Investigating Terephthalate Biodegradation: Structural Characterization of a Putative Decarboxylating cis-Dihydrodiol Dehydrogenase. Journal of Molecular Biology 423: 284–293.